mRNA Synthesis CRO Services-Enorna Biotechnology

Workflow and Advantages|Services|Testing Panel

Enorna simplifies your preclinical development process through our flexible mRNA synthesis services.

Our end-to-end solutions—spanning from sequence design and gene synthesis to in vitro transcription (IVT)—can deliver research-grade mRNA products in as little as 3 weeks, providing robust support for both in vitro and in vivo studies.

Workflow and Advantages

Gene Synthesis

(Optional)

Plasmid Preparation

Plasmid Linearization

In Vitro Transcription (IVT)

Why Us

1Technically Superior mRNA

•dsRNA controlled to minimize immune activation

•Free-to-operate backbone available

•Tailored testing packages

2Cost-Efficient without Quality Compromise

•Bulk discounts for multi-project deals

•Value-added services – free consultations

3IP Solution Provider

•Patent-protected alternatives: Our proprietary caps, enzymes, and LNPs come with clear IP ownership, eliminating legal uncertainties.

•Affordable access: Transparent licensing fees, no hidden royalties.

•Drop-in replacements: Compatible with industry-standard

4Scalable & Phase-Ready Manufacturing

•Seamless scale-up: GLP-grade batches up to 100 mg, GMP-grade CDMO services beyond

•Consistent quality: smooth transition from pre-clinical to clinical

mRNA CDMO Solutions

Services

We provide research grade and GLP grade mRNA synthesis services for both in vitro and in vivo studies.

Research Grade GLP Grade: RNA Type

mRNA, saRNA

mRNA, saRNA; Sequence Length*

0-15 kb

0-15 kb; Scale

1-10 mg

20-100 mg; Turnaround Time

7-10 Business Days

10-12 Business Days; Deliverable

a) Individual Cryotubes

b) ROA

a) Individual Cryotubes

b) ROA

*Additional fee may be charged for sequence longer than 7 kb.

Customizable Options

5’ Cap Options

Co-transcriptional capping：

• Partner-supplied Cap (Cap 1 structure)

• AG

• AG (3' OMe)

• AU

• Proprietary AG analog (FTO- guaranteed, licensable IP)

Enzymatic capping：Vaccine capping system
Modified NTP Options

• m1ψ-UTP• m5C

• ψ-UTP• m6A

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Testing Panel

QC

Item

Method

Standard

Research Grade

Preclinical Grade
Identification

Appearance

Visual Inspect

Clear and free of foreign particles

RNA length and intergrity

Agarose Gel Electrophoresis

Expected size band detected

Poly A length

Enzyme digestion to LC-MS

Target ± 5%

RNA content

UV Absorbance

Target ± 5%

pH

pHmeter

Target ± 0.5

Buffer Spefication

Client Spec

N/A
Purity

A260/280

UV Spec

1.70 ~ 2.30

Capping efficiency

LC-MS

≥ 90%

Size based purity

Capillary Electrophoresis

<4k nt: ≥75%

>4k nt: report value
Impurity

Total protein residue

Nano Orange Assay

≤1%

Plasmid DNA residue

qPCR

≤0.05%

dsRNA

ELISA

≤0.1%
Safety

Endotoxin

Semiquantitative

< 10EU/mg

Bioburden

Direct inoculation

No Growth after 72 hrs

Optional

Resources

Introduction to Enorna

Download

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mRNA Synthesis CRO Services

From Discovery to GLP—Optimized Speed, Quality, and Scale

We provide research grade and GLP grade mRNA synthesis services for both in vitro and in vivo studies.

5’ Cap Options

Modified NTP Options